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Angiogenesis Models
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Human skin graft model There are a number of commercially-available preclinical models of angiogenesis; however, Epistem has developed a novel approach for the assessment of anti-angiogenic agents based upon human skin xenografts. Following engraftment of human skin, the human endothelium is maintained within the graft and over 4-12 weeks angiogenesis occurs, increasing the number of human blood vessels. The host species endothelium also migrates into the graft and the number of vessels increase over the same period |
 Image: Human skin graft showing CD-31 positive blood vessels (anti-human) |
The effect of therapeutic agents on both human and host angiogenesis can be assessed.
Readouts
- Angiogenesis is measured using CD-31 immunohistochemistry to visualise vascularisation
- Blood vessel number and area are quantitatively measured using the ARIOL® image analysis system .
Future development of this assay will include the allografting of a tumour into the skin graft to measure the effect of tumour driven angiogenesis.