Gene Expression Analysis
Epistem Pharmacogenomics provides global and focused GCLP accredited mRNA and miRNA gene expression profiling and data analysis services.
Epistem Pharmacogenomics offers both Affymetrix and Agilent platforms enabling accurate expression analysis of the whole transcriptome. Sponsors can choose from pre-designed or custom designed arrays. Whole genome array services are available for human, non-human primate, dog, rat and mouse species.
Next Generation Sequencing (NGS)
Epistem Pharmacogenomics offer RNA and DNA sequencing on Illumina NGS platforms.
Epistem Pharmacogenomics offer focused Real-Time quantitative and qualitative PCR, with assays being designed and validated for industry standard performance based on a comparative Ct method.
Epistem Pharmacogenomics undertake analysis of data by:
- Unbiased interrogation of genes differentially expressed between the relevant comparison groups and subsequent biological interpretation
- Focused analysis of the behaviour of genes or pathways of interest if appropriate.
Standard analysis will include comprehensive statistical analysis and ANOVAs based on group experimental factors. We conduct biological interpretation and pathway analysis using specialised software packages which enable the accurate interpretation of the biological meaning in genomics data. We also use the Broad Institute Connectivity map that links gene patterns associated with disease to corresponding patterns produced by drug candidates. We also specialise in deciphering gene expression information to obtain gene expression signatures relevant to specific compounds that can be used in clinical settings to monitor drug treatment.