Inflammation

Psoriasis Model

Epistem offers clinically relevant in vitro and in vivo psoriasis models. Psoriasis is a common inflammatory disorder affecting the skin, characterised by inflamed and itchy plaques.

In vitro Psoriasis Model Description

Human primary keratinocytes are stimulated with a cocktail of pro-inflammatory cytokines to induce a gene expression profile which mimics that of psoriasis patients. The effects on gene expression in response to novel therapeutic agents can be assessed.

Readouts

  • Gene Expression
  • Protein Expression

In vivo psoriasis: imiquimod-induced psoriasis

Epistem also offers as in vivo psoriasis model.  The model is acute, and reflects inflammatory and epithelial changes which occur in psoriatic human skin.

Imiquimod-induced Model Description

Psoriasis is induced after topical application of 5% imiquimod cream.  Disease is assessed over a 5-8 day period using a Psoriasis Activity Severity Index (PASI) scoring system encompassing redness, swelling and thickness of the skin.  The effect of novel therapeutic agents can be assessed in-life and using the ex vivo analyses detailed below.

Readouts

  • FACS analysis of inflammatory mediators and key cytokines
  • Histology and immunohistochemistry
  • Multiplex analysis of key cytokines and factors
  • Gene Expression

 

Illustration of the effects of psoriasis

Illustration of the effects of psoriasis