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Inflammatory Bowel Disease Models

Epistem's preclinical  inflammatory bowel disease (IBD) platform is an integrated offering combining a comprehensive range of models with a high level of scientific experise. Epistem has a track record of delivering high quality preclinical results across a wide range of pharmaceutical clients.

Established models

Acute Models
Our standard acute models of chemically-induced disease are more rapid to complete, providing a shorter treatment period of 4-7 days.

  • DSS
  • TNBS

Chronic Models
Our range of models includes the CD4+CD62L+ adoptive T-cell transfer, which gives a reproducible chronic colitis.  Having more similarity to human disease than models of chemically-induced colitis, it permits the administration of test items in a therapeutic context over 2-3 weeks.

Model Development
Epistem is currently developing and validating its range of models to include:

  • Chronic DSS
  • Oxazolone

Typical parameters measured:

  • Body weight
  • Diarrhoea severity, faecal blood and overt rectal bleeding
  • Colon weight & length
  • Colitis severity score
  • Histometric quantification of epithelial loss and submucosal alterations
  • Myeloperoxidase (MPO)
  • Gene expression
  • Protein expression by immunohistochemistry
 
Typical IBD data from acute and chronic models
Correlation between MPO activity and colitis in the acute TNBS model
Correlation between MPO activity and colitis
in the acute TNBS model
Group diarrhoea and faecal blood occult incidence in the DSS model of IBD
Group diarrhoea and faecal blood occult incidence in the acute DSS model
Diarrhoea onset in the chronic T-cell transfer model
Diarrhoea onset in the chronic T-cell transfer model 
Colitis Severity in the T-cell transfer model
Colitis severity in the chronic T-cell transfer model