Epistem's gene expression service is a powerful tool providing highly sensitive analysis on human samples where low levels of RNA exist and conventional methods are not readily compatible:

• Single cells
• Single hair follicle
• 100 µl blood
• Endoscopic pinch biopsies

To increase sensitivity and allow processing of small samples, total cDNA is synchronously amplified from small amounts of purified RNA using Epistem’s proprietary technology.

This provides an accurate representative copy of the starting mRNA from which quantitative analysis can be undertaken using;

• qRT-PCR
• cDNA display
• cDNA array

The amplified cDNA is infinitely renewable through additional rounds of PCR, thereby providing a permanent cDNA archive.