Epistem has developed techniques for reliable and reproducible protein quantification in single plucked human hairs by immunohistochemistry and ELISA. Protein expression and site-specific phosphorylation can be detected and quantified using either whole mount or longitudinal sectioning techniques. Labelling protocols and quantification methods can be developed for each antigen. Using Epistem's ex vivo culture technique direct engagement of therapeutic target pathways can be demonstrated at the preclinical stage in our GCLP-compliant histology labs and translated to clinical study design.

 

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