Epistem provides a Dextran Sulfate Sodium (DSS)-mediated model of acute colitis. This chemically-induced acute DSS model exhibits components of the innate and adaptive immune system and is most responsive to immunosuppressive agents.

Acute DSS Model Description

Colitis is induced by administering DSS in the drinking water. This model typically runs for 7 days and is characterised by an acute diarrhoeal response, mucosal bleeding and delayed body weight loss. Colitis is defined by discontinuous lesions in the large bowel with areas of epithelial crypt loss, ulceration and infiltration of myeloid and lymphoid cells into the mucosa.

Primary Readouts

  • Large bowel histopathology

Further Readouts

  • Large bowel weight : length ratio
  • Immunohistochemistry and immunofluorescence
  • Physiological
  • Weight loss
  • Diarrhoea severity
  • Mucosal bleeding / occult blood
  • FACS analysis of inflammatory cell response
  • Multiplex analysis of serum and tissue cytokines
  • Protein expression
  • Gene expression

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H&E staining of a normal colon

H&E staining of a normal colon

H&E staining of DSS-induced diseased colon

H&E staining of DSS-induced diseased colon

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