Epistem provides a Dextran Sulfate Sodium (DSS)-mediated model of acute colitis. This chemically-induced acute colitis model exhibits components of the innate and adaptive immunity and is most responsive to immunosuppressive agents.
Acute DSS Model Description
Colitis is induced by administering DSS in the drinking water. This model typically runs for 7 days and is characterised by an acute diarrhoeal response, mucosal bleeding and delayed body weight loss. Colitis is defined by discontinuous lesions in the large bowel with areas of epithelial crypt loss, ulceration and infiltration of myeloid and lymphoid cells into the mucosa.
- Large bowel histopathology
- Large bowel weight : length ratio
- Immunohistochemistry and immunofluorescence
- Weight loss
- Diarrhoea severity
- Mucosal bleeding
- FACS analysis of inflammatory cell response
- Multiplex analysis of serum and tissue cytokines
- Protein expression
- Gene expression
- Intestinal permeability assay (FITC Dextran)
- Fecal Lipocalin (Lcn-2) assay
Chronic DSS Model Description
The DSS-induced model of colitis can also be modified to offer a chronic version using repeated cycles of exposure to DSS.