Epistem has extensive experience delivering other IBD models with requirements  to deliver specific data, within the framework of APSA policy and NC3Rs guidance.

Our legacy portfolio includes the following models:

2, 4, 6-trinitrobenzene sulphonic acid (TNBS)-mediated model of acute colitis

Like the DSS IBD model, this chemically-induced acute TNBS model exhibits components of the innate and adaptive immune system. Colitis results from impaired epithelial barrier function and sensitisation to luminal antigens. The model is characterised by acute weight loss, initial diarrhoea, stenosis and stool compaction. Colitis is focused within the mid and distal large bowel and is responsive to locally-acting steroids.

CD8(+)CD62L(+) T cell transfer-mediated colitis

Adoptive transfer of naïve cytotoxic T cells also causes a colitis, with similar disease presentation to colitis induced by the adoptive transfer of naïve CD4 T cells, although over a longer time course of up to 9 weeks.

 

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H&E staining of moderate TNBS-induced diseased colon

H&E staining of moderate TNBS-induced diseased colon

H&E staining of severe TNBS-induced diseased colon

H&E staining of severe TNBS-induced diseased colon

Immunofluorescent imaging of CD8 T cells in the colonic mucosa

Immunofluorescent imaging of CD8 T cells in the colonic mucosa

H&E stained transverse section through the mid-colon, demonstrating epithelial hyperproliferation and inflammatory cell infiltrate in mucosa and sub-mucosa

H&E stained transverse section through the mid-colon, demonstrating epithelial hyperproliferation and inflammatory cell infiltrate in mucosa and sub-mucosa

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Bespoke Colitis Models?

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Contact us via

info@epistem.co.uk

+44 (0)161 850 7600

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