Mice deficient in IL-10 develop colitis spontaneously over time, unless they are maintained under germ-free conditons. The pathology is similar to that observed in T cell transfer models, with a Th1/Th17-type immune response and epithelial hyperplasia, mixed inflammatory cell infiltrate in the mucosa and submucosa, crypt abscess formation and occasional ulceration; in addition, formation of cryptopatches can be observed.

Primary Readouts

  • Large bowel histopathology

Further Readouts

  • Large bowel weight : length ratio
  • Immunohistochemistry and immunofluorescence
  • Diarrhoea severity
  • Weight loss
  • FACS analysis of inflammatory cell response
  • Multiplex analysis of serum and tissue cytokines
  • Protein expression
  • Gene expression
  • Intestinal permeability assay (FITC Dextran)
  • Fecal Lipocalin (Lcn-2) assay

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IL-10 Knockout Spontaneous Colitis?

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