Epistem offers an adoptive T-cell transfer-mediated model of chronic colitis. This model is dependent on adaptive immunity and is suitable for the evaluation of therapies that can be administered either prophylactically or therapeutically.

Adoptive T-Cell Transfer Model Description

Colitis is induced by transferring naïve CD4+CD62L+ T-cells from donors to recipients. Disease results from the activation of donor T-cells by luminal antigens. This adoptive T-cell transfer model typically runs over 4-5 weeks. Colitis is associated with a Th1/Th17-type immune response and is characterised by epithelial hyperplasia, goblet cell depletion and infiltration of inflammatory cells into the mucosa and sub-mucosa; crypt abscess formation and ulceration may also be observed.

Several combinations of donor and recipient mouse strains are available, including:
1) Donor: BALB/c, Recipient: CB17/Icr-Prkdcscid/IcrlcoCrl
2) Donor: GM C57BL/6J, Recipient B6.Cg-Prkdcscid/SzJ
3) Donor: C57BL/6NTac, Recipient: B6.129S6-Rag2tm1Fwa N12
Discuss with our team to establish the model with new strains.

Primary Readouts

  • Large bowel histopathology

Further Readouts

  • Large bowel weight : length ratio
  • Immunohistochemistry and immunofluorescence
  • Diarrhoea severity
  • Weight loss
  • FACS analysis of inflammatory cell response
  • Multiplex analysis of serum and tissue cytokines
  • Protein expression
  • Gene expression
  • Intestinal permeability assay (FITC Dextran)
  • Fecal Lipocalin (Lcn-2) assay
  • Tcell engraftment flow cytometry confirmation

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H&E staining of normal colon

H&E staining of normal colon

H&E staining of diseased colon following CD4+CD62L+ T-cell transfer

H&E staining of diseased colon following CD4+CD62L+ T-cell transfer

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