Epistem offers orthotopic models of Acute Lymphoblastic Leukemia (ALL).  Human leukemic cell lines are intrafemoraly injected to ensure consistent engraftment and development of disease at the clinically applicable site. Intravenous injection of leukemic cells is also available.

Epistem's orthotopic Acute Lymphoblastic Leukemia model uses the SEM cell line. The karyotype of the SEM cell line is most common in children under 12 months of age and is characterised by the overproduction and lack of differentiation of lymphocytes. This cell line has been lentivirally transduced with luciferase and GFP allowing in-life disease monitoring using optical imaging.

Acute Lymphoblastic Leukemia Description

Cells (SEM) expressing luciferase and GFP are transplanted intrafemorally. Engraftment is detected by optical imaging on average, by day 7 and systemic disease can be typically observed by day 14.

Epistem's Cell Lines:

  • SEM

Primary Readout

  • Leukemic burden by optical imaging

Further Readouts

  • FACS analysis
  • Complete blood counts 
  • Body weight and condition
  • Serology

Leukemia cells

Leukemia cells

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