Psoriasis Model

Epistem offers clinically relevant in vitro and in vivo psoriasis models as a service. Psoriasis is a common inflammatory disorder affecting the skin, characterised by inflamed and itchy plaques.

In vitro Psoriasis Model Description

Human primary keratinocytes are stimulated with a cocktail of pro-inflammatory cytokines to induce a gene expression profile which mimics that of psoriasis patients. The effects on gene expression in response to novel therapeutic agents can be assessed.

Readouts

Gene Expression
Protein Expression

In vivo psoriasis: imiquimod-induced psoriasis

Epistem also offers as in vivo psoriasis model. The model is acute, and reflects inflammatory and epithelial changes which occur in psoriatic human skin.

Imiquimod-induced Model Description

Psoriasis is induced after topical application of 5% imiquimod cream. Disease is assessed over a 5-8 day period using a Psoriasis Activity Severity Index (PASI) scoring system encompassing redness, swelling and thickness of the skin. The effect of novel therapeutic agents can be assessed in-life and using the ex vivo analyses detailed below.