Epistem offers clinically relevant in vitro and in vivo psoriasis models as a service. Psoriasis is a common inflammatory disorder affecting the skin, characterised by inflamed and itchy plaques.
In vitro Psoriasis Model Description
Human primary keratinocytes are stimulated with a cocktail of pro-inflammatory cytokines to induce a gene expression profile which mimics that of psoriasis patients. The effects on gene expression in response to novel therapeutic agents can be assessed.
Readouts
- Gene Expression
- Protein Expression
In vivo psoriasis: imiquimod-induced psoriasis
Epistem also offers as in vivo psoriasis model. The model is acute, and reflects inflammatory and epithelial changes which occur in psoriatic human skin.
Imiquimod-induced Model Description
Psoriasis is induced after topical application of 5% imiquimod cream. Disease is assessed over a 5-8 day period using a Psoriasis Activity Severity Index (PASI) scoring system encompassing redness, swelling and thickness of the skin. The effect of novel therapeutic agents can be assessed in-life and using the ex vivo analyses detailed below.
Readouts
- FACS analysis of inflammatory mediators and key cytokines
- Histology and immunohistochemistry
- Multiplex analysis of key cytokines and factors
- Gene Expression
Illustration of the effects of psoriasis