Plucked hair follicles from patients with Chronic Discoid Lupus Erythematosus (CDLE) display a disease-specific genomic molecular signature
This pilot study showed that a disease-specific gene signature for Chronic Discoid Lupus Erythematosus can be obtained from plucked hair follicles followed by gene expression analysis.
In vivo selection reveals autophagy promotes adaptation of metastatic ovarian cancer cells to abdominal microenvironment
An increase in autophagic potency among ovarian cancer cells may be crucial for selection of metastatic colonies in the abdominal microenvironment. Autophagy may be a promising potential therapeutic target in the treatment of ovarian cancer.
Spatial metagenomic characterisation of microbial biogeography in the gut
Evaluation of a novel technique, MaPS-seq, a multiplexed protocol combining the positive aspects of metagenomics, transcriptomics and imaging to analyse the gut microbiome.
Identification of a Radiosensitivity Molecular Signature Induced by Enzalutamide in Hormone-sensitive and Hormone-resistant Prostate Cancer Cells
Androgen Deprivation Therapy (ADT) has demonstrated improved clinical success with further anti-tumour efficacy when combined with XRT, however, half of patients develop a resistance. In this study the authors identified potential prognostic biomarkers for response to combined ENZA, ADT and XRT therapy.
Defining disease mechanism and probing for effective treatments for Generalized Pustular Psoriasis by RNA-seq transcriptomics profiling
To investigate GPP pathogenesis and the efficacy of drugs on molecular level, Wang et al performed RNA-sequencing on peripheral blood mononuclear cells (PBMCs) obtained from five adult GPP patients before and during treatment with the oral retinoid acitretin.
Distinct Immune Cell Populations Define Response to Anti-PD-1 Monotherapy and Anti-PD-1/Anti-CTLA-4 Combined Therapy
To discover potential biomarkers of response and resistance to anti-PD1 monotherapy and combined anti-PD1/anti-CTLA-4 immunotherapies in a cohort of melanoma patients.
Next Generation Sequencing versus Microarray – a Comparison Using a PI-3K Inhibitor
Epistem are pleased to announce their acquisition of the Illumina NextSeq 550 next generation sequencer. With tunable output and high data quality, it provides the flexible power you need for whole-genome, transcriptome, and targeted resequencing.
Hedgehog stimulates hair follicle neogenesis by creating inductive dermis during murine skin wound healing
Mammalian adult skin wounds typically heal by fibrotic repair without hair follicle (HF) regeneration. This study is the first to show that de novo HFs can be regenerated in adult skin by modulating a Shh pathway in the dermis.
Redefining the practical utility of blood transcriptome biomarkers in inflammatory bowel disease
Screening for IDB candidate genes using RNA sequencing of whole blood liquid biopsies taken from relatively large cohorts of both adult and pediatric patients with defined clinically active IBD.
Epithelial-mesenchymal transition in human prostate cancer demonstrates enhanced immune evasion marked by IDO1 expression
Cancer invasion and metastasis are driven by epithelial-to-mesenchymal transition (EMT). This study identifies an important link between EMT and immune evasion in prostate cancer patients.
Transcriptomic analysis of hepatocellular carcinoma reveals molecular features of disease progression and tumour immune biology
Hepatocellular carcinoma (HCC) is a common cause of cancer deaths with a particularly poor prognosis. A recent publication by Okrah et al., describing molecular aspects of HCC progression illustrates the power of multigene transcriptomic signatures suggesting a rationale for immunotherapy early in HCC disease progression and potentially in combination with an anti-fibrotic strategy.
A stromal cell population that inhibits adipogenesis in mammalian fat depots
Adipocyte development and differentiation play an important role in the origin of obesity and many related diseases such as type 2 diabetes and cancer. Using a single-cell transcriptomics-based approach in a mouse model revealed three different adipocyte progenitor cell populations, including one subpopulation, termed Aregs, that suppressed adipocyte formation in vitro and in vivo.
Gene expression profiles from plucked scalp hair to assess pharmacodynamic biomarkers following treatment of advanced solid tumors with a novel anti-cancer stem cell Wnt inhibitor
Wnt signaling pathway genes are crucial for cell fate determination and cell polarity during development. Ipafricept (OMP-54F28) is a first in class recombinant fusion protein producing a truncated decoy receptor that binds to a cystine-rich region of Wnt ligand and as a result it blocks the activation of the Wnt signaling. Here the authors described the results of a phase 1 first in-human clinical trial.
Improved healing of large wounds via the reprogramming of wound-resident mesenchymal cells into epithelial cells
The authors of this paper demonstrated that mesenchymal cells within a large wound could be converted into keratinocytes in vivo via the transduction of four genes. This improved re-epithelialization of wounds through generation of new epithelial tissue that showed normal morphology/characteristics and eventually connected to host skin. The paper provides proof of principle for a new wound healing treatment particularly useful for large wounds that normally require skin grafts.
Oncogenic JAK2V617F causes PD‑L1 expression, mediating immune escape in myeloproliferative neoplasm
Oncogenic JAK2V617F causes PD-L1 expression, mediating immune escape in myeloproliferative neoplasms
New orthotopic organoid xenograft model enables reconstruction of human colon in vivo
A recent Cell Stem Cell paper describes a novel technique to orthotopically engraft human colon organoids in vivo. They observed long-term full reconstruction of the human colon upon engraftment, self-renewal of human Lgr5+ stem cells and multi-lineage differentiation. This model allows investigation of human stem cell functionality. Indeed, data presented demonstrates cell-intrinsic control of proliferation and differentiation within the human tissue, with slower rates of stem cell cycling observed than that of the host.
Stratification of Patients using Gene Expression Analysis of Laser Capture Microdissected (LCM) Tissue and Histology/IHC
This procedure could help stratify patients where pre-treatment could be avoided, these patients may then benefit from advanced surgery or alternative treatment options and prevent unnecessary toxicities.
Gene expression analyses of murine skin highlight strain-dependent effects of Imiquimod on psoriatic signatures
Our gene expression analysis and detailed protein analyses by flow cytometry and Luminex® can further assess disease pathogenesis by the identification of key molecules, or the intracellular mediators they express (cytokines, transcription factors).
JAK2, a novel therapeutic target in lung adenocarcinoma?
The field of personalised medicine to provide understanding of the molecular basis of disease is constantly growing. Multiple signalling pathways have been identified that associate with malignant transformation. However, the vast majority of lung cancer patients have no known driver genes detected, and they are still treated with standard cytotoxic chemotherapy.
Development of a Signature of Aberrant Myc Transcription has Prognostic Value in Evaluating Novel Myc-Targeted Therapies
Altered expression of transcription factors are key indicators in cancer progression in functions such as proliferation and differentiation, angiogenesis and apoptosis. A significant proportion of cancers are driven by mutations in these networks, including Wnt/β-catenin, Ras/MAPK and Myc
Laser Capture Microdissection for Gene Expression Analysis in Distinct Cell Populations
Knowledge in early phase cancer development to provide understanding of the mechanism of action of oncogenic mutations is constantly growing. A recent study carried out by Mo et al has highlighted several interesting results.
Plucked hair ex vivo model identifies novel Notch PD biomarker suitable for use in the clinic.
Epistem have validated plucked scalp hair as a robust ex vivo model allowing biomarker signature identification. Readouts in these models include expression profiling by microarray or RNA-Seq, and targeted assays by qPCR.