The importance of Reg4 in the response of the colonic epithelium to inflammation

Upregulation of, or treatment with Reg4 was associated with the maintenance and proliferation of colonic organoid cultures, the epithelial response to mucosal injury in an in vivo model of chemically-induced acute colitis (DSS) and in paediatric patients with Intestinal Failure and the response of intestinal epithelial cells to an inflammatory stimulus (LPS) in vitro.

Prophylactic TNF blockade uncouples efficacy and toxicity in dual CTLA-4 and PD-1 immunotherapy

Prophylactic TNF blockade may safely allow doses of ipilimumab to be increased in immune checkpoint blockade combined therapies.

Redefining the practical utility of blood transcriptome biomarkers in inflammatory bowel disease

Screening for IDB candidate genes using RNA sequencing of whole blood liquid biopsies taken from relatively large cohorts of both adult and pediatric patients with defined clinically active IBD.

The influence of TLR5 expression by intestinal epithelial cells on the gut microbiome

It has been shown that TLR5 expression by the intestinal epithelium early in neonatal development is an important factor in preventing increased colonisation by flagellated bacteria, which can drive chronic pathologies such as metabolic syndrome and colitis

The relationship between epithelial cell fate, microbiota composition and inflammation in the intestine

How perturbation of the homeostatic mechanisms governing epithelial cell fate in the intestine influence the gut microbiota and the susceptibility to colitis. The influence of structural proteins on key signalling pathways and their downstream transcriptional machinery are discussed.

CCDC88B and its Importance in Inflammatory Bowel Disease

Epistem have extensive experience of running in vivo colitis models including off-the-shelf models of adoptive T cell transfer-mediated and DSS-induced acute colitis; in addition, we are happy to develop and run bespoke models in collaboration with Sponsors.

Adoptive T Cell Transfer Models of Colitis

This model offers the ability to study test items using a range of dosing regimens, from dosing in a prophylactic manner through to examining efficacy in ameliorating early stage or established disease.

CCL20 as a biomarker of drug efficacy in IBD

The chemokine CCL20 (Mip-3α) plays an important role in recruiting Th17 cells (which express the corresponding receptor, CCR6) to sites of inflammation, thus playing a role in the pathogenesis of chronic inflammatory conditions such as Crohn's disease. In a recent paper in the Journal of Crohn's and Colitis, Giovanni Monteleone and colleagues presented data from clinical and laboratory studies on Mongersen, a Smad7 antisense oligonucleotide.

Accessing Disease End-Points in IBD Models

High quality histopathology analysis is standard for all our IBD service models. In addition, Epistem offer a wide range of analytical services that allow Sponsors to acquire quantitative experimental data for a variety of disease-relevant end-points.

New paper demonstrating the microbiota impact on cell proliferation via microRNA expression changes in intestinal epithelial stem cells

Intestinal flora are known to impact the immune system and metabolism, with chronic exposures to certain flora linked to conditions such as inflammatory bowel disease and fibrosis. Breeches of the epithelial barrier and systemic infection can lead to more acute problems such as sepsis.

New insights into epithelial barrier dysfunction in the intestine: relevance for IBD

Alterations in the complex inter-relationship between intestinal epithelial cells, the mucosal immune system responses and the gut microbiota are responsible for the development of inflammatory bowel disease in humans.