The serotonin reuptake transporter is reduced in the epithelium of active Crohn’s disease and ulcerative colitis

Our team recently came across an IBD study which demonstrated for the first time that the serotonin reuptake transporter protein is downregulated in inflamed intestinal tissue.

ACE2 contributes to the maintenance of mouse epithelial barrier function

A recent COVID-19 study has utilised organoids to look into the effects of deleting ACE2, the protein which the virus uses to enter the cell.

CD47 is a negative regulator of intestinal epithelial cell self-renewal following DSS-induced experimental colitis

Scientists have demonstrated that epithelial cell CD47 expression in the colon is strongly induced in patients with ulcerative colitis and Crohn's disease.

Reducing intestinal inflammation with secondary bile acids

Intestinal inflammation was shown to be associated with reduced levels of secondary bile acids and bacteria of the Ruminococcaceae family in a recent study moving onto clinical trials.

Flagellin-elicited adaptive immunity suppresses flagellated microbiota and vaccinates against chronic inflammatory diseases

A recent study has demonstrated positive in vivo results for a flagellin-based vaccine for IBD treatment.

Spatial metagenomic characterisation of microbial biogeography in the gut

Evaluation of a novel technique, MaPS-seq, a multiplexed protocol combining the positive aspects of metagenomics, transcriptomics and imaging to analyse the gut microbiome.

The importance of Reg4 in the response of the colonic epithelium to inflammation

Upregulation of, or treatment with Reg4 was associated with the maintenance and proliferation of colonic organoid cultures, the epithelial response to mucosal injury in an in vivo model of chemically-induced acute colitis (DSS) and in paediatric patients with Intestinal Failure and the response of intestinal epithelial cells to an inflammatory stimulus (LPS) in vitro.

Prophylactic TNF blockade uncouples efficacy and toxicity in dual CTLA-4 and PD-1 immunotherapy

Prophylactic TNF blockade may safely allow doses of ipilimumab to be increased in immune checkpoint blockade combined therapies.

Redefining the practical utility of blood transcriptome biomarkers in inflammatory bowel disease

Screening for IDB candidate genes using RNA sequencing of whole blood liquid biopsies taken from relatively large cohorts of both adult and pediatric patients with defined clinically active IBD.

The influence of TLR5 expression by intestinal epithelial cells on the gut microbiome

It has been shown that TLR5 expression by the intestinal epithelium early in neonatal development is an important factor in preventing increased colonisation by flagellated bacteria, which can drive chronic pathologies such as metabolic syndrome and colitis

The relationship between epithelial cell fate, microbiota composition and inflammation in the intestine

How perturbation of the homeostatic mechanisms governing epithelial cell fate in the intestine influence the gut microbiota and the susceptibility to colitis. The influence of structural proteins on key signalling pathways and their downstream transcriptional machinery are discussed.

CCDC88B and its Importance in Inflammatory Bowel Disease

Epistem have extensive experience of running in vivo colitis models including off-the-shelf models of adoptive T cell transfer-mediated and DSS-induced acute colitis; in addition, we are happy to develop and run bespoke models in collaboration with Sponsors.

Adoptive T Cell Transfer Models of Colitis

This model offers the ability to study test items using a range of dosing regimens, from dosing in a prophylactic manner through to examining efficacy in ameliorating early stage or established disease.

CCL20 as a biomarker of drug efficacy in IBD

The chemokine CCL20 (Mip-3?) plays an important role in recruiting Th17 cells (which express the corresponding receptor, CCR6) to sites of inflammation, thus playing a role in the pathogenesis of chronic inflammatory conditions such as Crohn's disease. In a recent paper in the Journal of Crohn's and Colitis, Giovanni Monteleone and colleagues presented data from clinical and laboratory studies on Mongersen, a Smad7 antisense oligonucleotide.

Accessing Disease End-Points in IBD Models

High quality histopathology analysis is standard for all our IBD service models. In addition, Epistem offer a wide range of analytical services that allow Sponsors to acquire quantitative experimental data for a variety of disease-relevant end-points.

New paper demonstrating the microbiota impact on cell proliferation via microRNA expression changes in intestinal epithelial stem cells

Intestinal flora are known to impact the immune system and metabolism, with chronic exposures to certain flora linked to conditions such as inflammatory bowel disease and fibrosis. Breeches of the epithelial barrier and systemic infection can lead to more acute problems such as sepsis.

New insights into epithelial barrier dysfunction in the intestine: relevance for IBD

Alterations in the complex inter-relationship between intestinal epithelial cells, the mucosal immune system responses and the gut microbiota are responsible for the development of inflammatory bowel disease in humans.