Integrative RNA-Seq and ChIP-Seq Analysis of Human Lung Cancer Cells Isolated by Laser Microdissection

A study looking at the development of a workflow for integrative RNA-sequencing (RNA-Seq) and chromatin immunoprecipitation sequencing (ChIP-Seq) using laser capture microdissected clinical samples of LUAD and LUSC.

Lgr5+ telocytes are a signalling source at the intestinal villus tip

A recent study utilising spatially-resolved transcriptomics and laser capture microdissection has discovered that Lgr5+ telocytes act as a signalling source in the villus tip of the intestine.

Enrichment of CpG island shore region hypermethylation in epigenetic breast field cancerisation

Scientists have demonstrated that normal tissue from the tumour harbouring breast is epigenetically different from normal tissue of the opposite breast despite histology appearing normal in both cases.

Heterogeneous components of lung adenocarcinomas harbour distinct EGFR mutations and PD-L1 expression

A recent study using laser capture microdissection demonstrated that different areas of the same lung adenocarcinoma tissue can harbour both sensitising EGFR mutations and those mutations that confer resistance to TKIs.

Epithelial-mesenchymal transition in human prostate cancer demonstrates enhanced immune evasion marked by IDO1 expression

Cancer invasion and metastasis are driven by epithelial-to-mesenchymal transition (EMT). This study identifies an important link between EMT and immune evasion in prostate cancer patients.

Overexpression of miR-221 and miR-222 in the cancer stroma is associated with malignant potential in colorectal cancer

In this study the authors used laser capture microdissection to investigate the role of stromal miRNA in patients with metastatic cancer, aiming to identify those miRNAs present in the stroma that may contribute to metastases.

Laser Capture Microdissection (LCM) - A Strategy for Success

Micro-sampling by laser capture microdissection is a powerful tool allowing specific analysis from whole tissues, in heterogeneous microenvironments such as tumours, where less well represented target cells are dwarfed by tissue stroma and other cell types. Whilst the application is potentially powerful for target discovery and mechanistic understanding, the process is notoriously difficult, particularly in clinical specimens. Our Laser Capture Microdissection team has evaluated the findings from all the studies run in our GCLP-accredited labs and devised a strategy for LCM studies maximising the potential of success and giving the sponsor control over go/no go decisions.

Stratification of Patients using Gene Expression Analysis of Laser Capture Microdissected (LCM) Tissue and Histology/IHC

This procedure could help stratify patients where pre-treatment could be avoided, these patients may then benefit from advanced surgery or alternative treatment options and prevent unnecessary toxicities.

Laser Capture Microdissection for Gene Expression Analysis in Distinct Cell Populations

Knowledge in early phase cancer development to provide understanding of the mechanism of action of oncogenic mutations is constantly growing. A recent study carried out by Mo et al has highlighted several interesting results.