Genome-wide epigenetic and transcriptomic characterisation of human-induced pluripotent stem cell-derived intestinal epithelial organoids

Recent developments have allowed us to generate epithelial cell-specific intestinal organoids from pluripotent stem cells, we review a study which aims to characterise these organoid cultures and compare them with cultures derived from primary intestinal epithelial cells.

5-Fluorouracil treatment induces characteristic T>G mutations in human cancer

Organoid models treated with 5-FU suggest further oncology scrutiny is needed when treating younger patients

Derivation of adult canine intestinal organoids for translational research in gastroenterology

This study describes the development and long-term maintenance of small intestine and colon in vitro canine organoids using whole tissue and biopsy samples from 40 healthy and diseased cohorts.

Human 3D Gastrointestinal Microtissue Barrier Function As a Predictor of Drug-Induced Diarrhoea

New preclinical approaches are required for earlier testing of therapies for GI-toxicity. In this study the authors describe an in vitro human 3D GI microtissue and validated it for GI-toxicity using a panel of known drugs that do/do not cause diarrhoea.

Can organoids guide clinical decisions? Current successes and potential pitfalls

A paper in the Nature Medicine journal reviews the use of organoids in the clinic up-to-date and also highlights some of the potential obstacles for widespread use as predictive tools for in vivo treatment. Our Senior Scientist, Dr Sarah Hoyle, summarises the key points from this paper.

Human organoids provide new insights into early stage enterovirus infection

Epistem have presented developmental work within the organoid model in the areas of epithelial regeneration and GI toxicity at various conferences. Potential readouts in this model could be proliferation, differentiation, apoptosis, cytotoxicity screening and protein and gene expression.

Organoids programmed to differentiate into rare hormone-producing enteroendocrine cells

New paper describes protocols for the manipulation of organoid Lgr5+ stem cells into quiescence before being programmed to differentiate into specific intestinal lineages, including the rare hormone-producing endocrine cell