Epistem's multiplex service employs a Luminex Bio-Plex® 200 System which utilises a bead-based immunoassay platform. Higher sensitivity and throughput than ELISA, multi-parameter readouts are possible using small sample volumes. Secreted proteins in blood products, lysed tissue and cell culture supernatants can all be assessed. Cytokines can be analysed using standard screening panels or custom built panels can be designed to target analytes of interest.

Epistem's multiplex service is available as a stand-alone analysis service or as part of a comprehensive integrated preclinical package.

Typical analytes include:

  • IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10
  • GM-CSF
  • TNF-alpha
  • Basic FGF
  • G-CSF
  • IFN-gamma
  • VEGF

Epistem's multiplex analysis service can be used to support in-house models, including those where tissue and plasma cytokine analysis is key, such as:

96 well plate

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