Next Generation Sequencing
Next Generation Sequencing (NGS) is a powerful technique which helps you get the most out of your study. Epistem's expertise with different sample types and low input amounts coupled with the Illumina NGS platforms offers a range of sequencing options for DNA and RNA sample analysis. For DNA samples, whole exome sequencing (WES) and custom targeted sequencing have become increasingly popular due to the ability to sequence samples with a much higher coverage and detect novel or rare variants. For RNA samples, whole transcriptome sequencing and small RNA sequencing can assess alterations in gene expression. Additionally, T cell receptor profiling is available with targeted RNA sequencing panels. Microbiome sequencing is available for customers wishing to investigate the bacterial diversity present in a given sample.
Epistem’s sequencing options include:
- Whole genome sequencing
- Whole exome sequencing
- Targeted resequencing (commercially available assays and customised assays)
- Multiple disease panels available
- Microbiome analysis
Epistem also offer dedicated bioinformatic analyses to suit your requirements.
Epistem undertakes analysis of data by:
- Unbiased interrogation of genes differentially expressed between the relevant comparison groups and subsequent biological interpretation
- Focused analysis of the behaviour of genes or pathways of interest
Standard analysis will include comprehensive statistical analysis and ANOVAs based on group experimental factors. We conduct biological interpretation and pathway analysis using specialised software packages which enable the accurate interpretation of the biological meaning in genomics data. We also use the Broad Institute Connectivity map that links gene patterns associated with disease to corresponding patterns produced by drug candidates. We also specialise in deciphering gene expression information to obtain gene expression signatures relevant to specific compounds that can be used in clinical settings to monitor drug treatment.