Microbiome Evaluation Panel
The microbiome refers to a group of microorganisms or their genomes found in an environmental niche. In humans there are approximately ten times as many microbial cells and 100 times as many microbial genes than there are human. These microbiota are found in places like mouth, gut, skin etc and can influence the expression of human genes in these areas even contributing to disease states such as IBD and diabetes. Advances in next generation sequencing has revolutionised the study of human and non-human microflora and we have several applications available at Epistem.
16s RNA Metagenomics Sequencing
We utilise library preparation kits suitable for 16s metagenomics sequencing on our NextSeq550 sequencer. We can sequence the V1-V4 hypervariable regions of the 16s RNA genes and even V1-9 hypervariable regions if required. Suitable for general microbiome assessments including skin and gut. Bioinformatics analysis is available for both alpha and beta diversity.
Next generation sequencing (NGS) has enabled assessment of many genomes from organisms in parallel. Whole genome sequencing of microbial DNA from any niche can give functional information and facilitate de novo genome assembly of genomes. NGS-based metagenomic sequencing can also detect low abundant genomes within a microbial community.
Metatranscriptome describes the complex expression of RNAs associated with a group of organisms in a sample from a microbial community. We can now sequence the metatranscriptome to gain important insights into many functions such as immune response, disease progression and antibiotic resistance.